When the channels, steps, and memories are fully configured, the sequencer can be run to execute the appropriate vectors.
Set the first step to also be the last step by checking the last step checkbox.
Set the Burst count to 1 and click the Run button to run the sequencer. The status of the run will be displayed in the status bar.

Run Sequencer
Relevant API functions:
● GtDio6xSequencerRun – Start execution of the burst starting a particular Step index.
● GtDio6xSequencerGetStatus – Read the sequencer status registers to determine the sequencer is still running.